Do I need to store the AquaGenomic kit in the refrigerator or freezer?
No, AquaGenomic Solution is stable at room temperature (~22 C). Vortex the AquaGenomic solution to mix well before dispensing. 2. Does AquaGenomic Solution contain Proteinase K? No. AquaGenomic can be used to extract DNA from cells and tissues without needing protease digestion. However, if mitochondrial DNA (mtDNA) recovery is desired, you will need to add Proteinase K to AquaGenomic solution to 100 mg/ml and incubate the sample at 60-65 C for 1-2 hours during cell lysis and then at 95 for 10 minutes to inactivate the Proteinase K. 3. Does AquaGenomic Solution contain RNase A? No. AquaGenomic Solution can remove most RNA contaminant. Trace amount of RNA would not interfere with most genomic DNA applications. However, if complete RNA removal is desired, RNase A (200 ug/ml) can be added to the AquaGenomic solution prior to extraction. 4. What type of homogenizer do you recommend for using with AquaGenomic? Pestle-and-tube homogenizers produce more homogeneous samples. However, if you do
