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Do I need to select for tet resistance when using DY380?

April 26, 2017dy380 resistance select tet

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Do I need to select for tet resistance when using DY380?

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No, it’s enough to select for the marker present on the plasmid/BAC in the bacteria. If growing DY380 up for preparation of competent cells (no vector), tet selection might be a good idea. Remember that EL350 and EL250 are not tet resistant.