Do I need to DNase treat my RNA before continuing on?
If you have not eliminated the chance of genomic amplification in your probe and primer design you will need to do a DNase treatment. We recommend you DNase treat the RNA on a column versus post RNA recovery. We have found the RNase free DNase set from Qiagen to perform well (cat#79254). RNA must be eluted in water (RNase and DNase free).
