Do I need to be careful with the type of chaotropic ion I use when eluting antibodies from affinity columns?
It is recommended to use the mildest chaotropic agents at the lowest possible concentration that will ensure rapid elution and high recovery of activity. Iodination reactions employing either chloramine T or IODO-GEN (1,3,4,6-tetrachloro-3-6-diphenylglycouril) are particularly sensitive to inhibition by low concentrations of thiocyanate ions and, if antibodies are eluted from affinity columns or Protein A affinity columns, using this chaotropic ion, it is essential that they are dialyzed thoroughly after elution to remove thiocyanate ions.
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