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Do I need to aspirate growth media from the cell wells prior to adding the Membrane Potential Assay Reagent?

April 26, 2017aspirate assay CELL growth media membrane potential prior Wells

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Do I need to aspirate growth media from the cell wells prior to adding the Membrane Potential Assay Reagent?

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No. If you do choose to aspirate growth medium, please maintain the volumes described in the protocol by backfilling the wells with an equal volume of buffer. Some cells, ion channels, and compounds are serum sensitive, so growth medium removal is necessary.