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Do I need to aspirate growth media from the cell wells prior to adding the Calcium Assay Reagent?

April 26, 2017aspirate assay calcium CELL growth media prior Reagent Wells

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Do I need to aspirate growth media from the cell wells prior to adding the Calcium Assay Reagent?

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No. The optimal protocol, as provided with the FLIPR Calcium Assay Kit, assumes no removal of growth media from the cells. The FLIPR Calcium Assay Kit was developed and optimized to allow the direct addition of the reconstituted reagents to the cell wells, so obtaining a 1:1 dilution of reagent in growth medium.