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Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?

April 26, 2017assays mirna Primers qPCR RT sequence step

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Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?

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No. Because RT² miRNA qPCR assay technology use universal reverse transcription in the RT step, the sequence specific miRNA primers do not need in this step. Therefore, cDNA obtained from universal RT can be used for any miRNA expression analysis.