Do I need special objectives for darkfield microscopy?
For reflected light darkfield observation, you need brightfield/darkfield objectives to do darkfield work. These objectives (labeled BD or BF/DF) function with the illuminator optics to keep the illuminating light separate from the image light of the specimen. In most cases, for transmitted light observation, you will only need a darkfield stop in the condenser. At higher magnifications, however, you will need an objective with an iris as well as a special darkfield condenser.
