Do I lose anything by using Python 2.3 versus newer Python versions, such as Python 2.5?
No. Django itself is guaranteed to work with any version of Python from 2.3 and higher. If you use a Python version newer than 2.3, you will, of course, be able to take advantage of newer Python features in your own code, along with the speed improvements and other optimizations that have been made to the Python language itself. But the Django framework itself should work equally well on 2.3 as it does on 2.4 or 2.5.