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Do I have to use retroviral packaging and infection to create stable cell lines?

April 26, 2017CELL create infection packaging retroviral STABLE

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Do I have to use retroviral packaging and infection to create stable cell lines?

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If your transfection efficiency is very high (e.g. 80% or greater), it is not necessary to use retroviral packaging. Simply split your cells 24hrs. post-transfection and add puromycin (0.5 – 1ug/ml) to the fresh growth medium.