Do I have to use poly-D-lysine coated plates when using the FLIPR Calcium Assay Kit?
With conventional calcium assay protocols, it is often necessary to seed mildly adherent or suspension cells into poly-D-lysine coated plates to improve adherence and minimize cellular blow-off. Depending on the individual cell line, with the FLIPR Calcium Assay Kit, poly-D-lysine coated plates may no longer be required. With HEK293 cells, we have comparable results from cells seeded in regular plates and poly-D-lysine coated plates when using the FLIPR Calcium Assay Kit. However, this may not hold true for all cell types. We therefore recommend optimization of the assay conditions for individual cell types.
