Do I have to remove standard antibiotics for the treatment with Mynox® ?
No. Standard antibiotics like penicillin/streptomycin could be carried along during the treatment withMynox®. As a basic principle, we would not recommend the use of antibiotics. Pen/Strep mainly effects germs of the mouth and faucal cavity and was introduced in times, when laboratory staff used their mouth for pipetting. This situation changed with the introduction of pipetting-aids and security workbenches. Antibiotics can effect the cellular metabolism and thus the results of experiments. (compare with: Kuhlmann, Cytotechnology 19:95-105,1996 “The prophylactic use of antibiotics in cell culture”). Bacterial contaminations can interfere latently and unrecognized. With Onar®EUB Minerva Biolabs provides a sensitive PCR-Detection Kit for bacterial contamination (Cat.-No. 12-1025). Mynox® was uneffective in eliminating mycoplasma from virus suspensions. The cell suspension must be free of cellular debris before treatment. Before the supernatant can be effectively treated, cellular debris
