Do I have to prepare a new gain reference image when rotating the DualVision cameras?
Yes, you do for the DV 300W. No, you do not for the DV 600. For DV300W, the scintillator and CCD are independent in their positions, so when rotation occurs, a new correspondence between the scintillator and the CCD pixel must be re-established. For DV600, the scintillator and the CCD remain a rigid body assembly, so camera rotation does not alter the relationship between the scintillator and the CCD.