Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Do I have any other alternative if I am not achieving complete cell lysis?

April 26, 2017achieving alternative CELL COMPLETE lysis

0

Posted

Do I have any other alternative if I am not achieving complete cell lysis?

1 Answer

0

Posted

A. Micro sonicator can be used to enable greater cell lysis. The process should be done with the tubes placed on ice in order to avoid sample overheating. Sonication is especially important for assays involving nuclear proteins such as chromatin and HDAC.