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Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?

April 26, 2017affect cloning Cycles freeze-thaw pdrive stability vector

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Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?

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No. Based on ligation and religation experiments, QIAGEN has not observed any instability of the U-overhang following multiple freeze/thaw cycles. Therefore, it is unnecessary to aliquot the pDrive Cl…