Do eukaryotic cells have restriction enzymes?
Restriction enzymes, also called restriction endonucleases, are enzymes that are used to cut DNA in the middle. Most restriction enzymes are found in prokaryotes, but there are several restriction enzymes found in eukaryotic cells, and they are normally called endonucleases. An example of an eukaryotic cell have has endonucleases is yeast, which helps prevent DNA damage from environmental agents. You can also say that DNA Gyrase has endonuclease activity, because they cut the DNA backbone in DNA replication to prevent supercoiling of the DNA (the extreme tension that is caused by unwinding the DNA).