Do enzymatic hydrolyzability and Simons stain reflect the changes in the accessibility of lignocellulosic substrates to cellulase enzymes?
In an attempt to elucidate the impact of substrate accessibility to cellulases on the susceptibility of lignocellulosic substrates to enzymatic hydrolysis, a hydrogen peroxide treated, Douglas fir kraft pulp was dried using several methods with varying levels of intensity. Oven-drying at 50 and 100 degrees C, air-drying, and freeze-drying methods were employed to remove the interfibrillar water from the pulp samples. Subsequently, the never-dried and variably dried pulps were hydrolyzed using a commercial cellulase preparation supplemented with additional beta-glucosidase. Drying reduced the susceptibility of the substrates to enzymatic hydrolysis, which can be attributed to the hornifying effect that drying has on fibers. This effect was more pronounced for the fibers that were oven-dried at 100 degrees C (23% reduction) and 50 degrees C (15% reduction), and there was a good correlation between the Simons’s stain results and the enzymatic digestibility of the dried pulps. These observ
Related Questions
- Do enzymatic hydrolyzability and Simons stain reflect the changes in the accessibility of lignocellulosic substrates to cellulase enzymes?
- Why do the changes done with any adjustment to the entitlement or scheme itself does not reflect in Leave Entitlement module or reports?
- Is the Omaha System periodically updated to reflect changes in practice, documentation, and information management?
