Do differences in expression patterns generated by different labeling methods give different results in dual-color comparative microarray analysis?
No, as long as both probes analyzed on the same slide have been prepared with exactly the same labeling method. Two labeling methods can be used side by side to identify exactly the same differential expression pattern, but these two labeling methods cannot be mixed. A labeling method that gives brighter probes, such as that used by the CyScribe Post-Labeling Kit, may give additional data, as low copy number transcripts become detectable.
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