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Do cells need to be sonicated and/or frozen before treatment with B-PER Reagent?

April 26, 2017b-per cells frozen Reagent sonicated treatment

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Do cells need to be sonicated and/or frozen before treatment with B-PER Reagent?

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B-PER Reagent is designed to replace sonication, which cannot fully recover soluble proteins or regain inclusion bodies. Although freezing the bacterial pellets is not usually necessary, it appears to increase protein yield in some bacteria that are traditionally difficult to lyse.