Do both Sulfhydryl-Reactive and Amine-Reactive kits perform equally well in all applications?
We have not tested all applications. Both kits have been optimized for flow cytometry as well as immunocytochemistry (ICC) and immunohistochemistry (IHC). Although both kits generate good results in these applications, we have found that the Sulfhydryl-Reactive kit may demonstrate a slight advantage for flow cytometry applications, and the Amine-Reactive kit may demonstrate a slight advantage for ICC/IHC applications but this will vary depending on the particular protein. It is critical for optimal performance, to use the recommended protocols and buffers as stated in our BestProtocols.
