Do blood samples need to be processed immediately?
Yes. Approximately 1-3 mL of blood samples freshly collected from a blood collection tube should be transferred immediately into one Vita-Cap™ N6 tube (each tube added 2-mL blood diluents containing anticoagulants) and subjected to cell enrichment by rotation at 10 rpm at 37°C for 3 hours for cancer cell attachment to occur. The rotation is best done laying the tubes between 2 bars on a rotator. The 10 rpm is the speed of the circular churning motion of the tube around its long axis.