Do any of the SureSilencing™ shRNA plasmids contain inducible promoters?
No, SureSilencing™ shRNA Plasmids with inducible promoters are not available. Inducible promoters are meant to control the timing of silencing, particularly for stable shRNA transfections for essential genes or for differentiation model systems. However, they tend to be too leaky for RNA interference. That is, they still express a certain amount of the shRNA of interest, even under repressed conditions. We do not recommend a specific vector system for this application. However, you are welcome to screen our shRNA sequences in another system, and then use them in your vector system of choice.
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- How do I download the Users Manual for the SureSilencing™ shRNA plasmids?
- Do any of the SureSilencing™ shRNA plasmids contain inducible promoters?
