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Do all chambers require superfusion of the vessel to maintain temperature and pH?

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Do all chambers require superfusion of the vessel to maintain temperature and pH?

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No, the LS-CH-1-SH, LS-CH-1-AU-SH, LS-CH-2-SH have an aluminum base heated by an electronic unit that sets and regulates the bath temperature when using buffers like HEPES or MOPS.

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