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Data Analysis Are controls spotted on the array?

April 26, 2017array controls data analysis spotted

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Data Analysis Are controls spotted on the array?

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• Yes, positive and negative controls are spotted in each sub-group on the array. • Positive control: Monoclonal Anti-Cy3/Cy5 antibodies or BSA labeled with Cy3/Cy5 • Negative control: Unlabeled BSA. • The .GAL file for antibody position and analysis spreadsheet can be found on the CD provided with the kit or may be downloaded from the Web site: sigma-aldrich.com/arrays. • Positive controls on the array are for orientation purposes and should not be used for data analysis calculations.