Customers detailed question: From an analytical point of view, under what circumstances would it be preferable to use a MycoSep® column for Trichothecenes clean up?
Whenever available MycoSep® clean up should be preferred: When the alternative would be liquid liquid partition the lower consumption of hazardous solvents and the huge reduction of working time is a clear advantage. Additionally, with MycoSep® clean up there are less sources for error. When the alternative method would be Immunoaffinity DON column, the MycoSep® clean up should be preferred due to the possibility of analysing other trichothecenes like Nivalenol, Fusarenon X, 3- and 15-Acetyldeoxynivalenol and the type A trichothecenes after MycoSep® clean up as well, which is not possible after Immunoaffinity clean up. Analysing the other fungal metabolites of this group gives a more complete picture of the contamination of the sample. The MycoSep® clean up also has the clear advantage of less time consumption: About 30 seconds for the MycoSep® clean up and about 30 minutes for an Immunoaffinity clean up. Only one reason is in favor of the Immunoaffinity column in this case: The Immuno
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