Cholesterol is a large molecule, is it stable as a stationary phase?
[top] Yes, the UDC Cholesterol phase has proven to be stable in 0.1% v/v TFA and 0.1% v/v H3PO4 at acid pHs and up through pH 8.00 with 20mM NH4COOH buffer with NH4OH. A pH in excess of pH 8.00 should be avoided as this phase has a mono dentate (single point) attachment to the silica matrix and an intermediate ester link in its structure. For higher pHs the Cogent Bidentate C18 is recommended.
