cells counted times depth factor times dilution factor divided by the number of 1mm2 squares counted What are reticulocytes and nucleated RBCs?
Reticulocytes are slightly immature RBCs that usually cannot be distinguished from mature RBCs on a Wrights-stained smear. Unlike mature RBCs, reticulocytes contain small clumps of ribosomal RNA. The ribosomal RNA can be stained with a supravital stain, such as brilliant cresyl blue or new methylene blue, so that the reticulocytes can be distinguished from mature RBCs. When stained with the supravital stains, the ribosomal RNA looks like blue strands or granules in the red blood cell. Equal amounts whole blood and stain are mixed and incubated. A smear is made and percentage of reticulocytes is obtained. Nucleated Red Blood Cells are immature RBCs. They are produced in the bone marrow and do not present themselves in the peripheral blood under normal conditions. The exception to this, may be the newborn infant. It is not uncommon to see a nucleated RBC (nRBC) on their peripheral smear. The “routine” number seen on a newborn’s peripheral is generally less that five per 100 WBC. The rele
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- cells counted times depth factor times dilution factor divided by the number of 1mm2 squares counted What are reticulocytes and nucleated RBCs?
- 180 divided by 2 to the 2 power times 10 to the 2 power +(190-145)
