Can you recommend the sonication conditions to obtain 300-500 bp fragments?
It is advised to optimize the shearing conditions “in house” with your own sample. Generally we recommend to use 300 µl of your DNA sample resuspended at 0.1 µg/µl (30 µg DNA) in 1,5 ml tube with the settings as below: • Bioruptor power: Low • cycles: 15 s ON and 15s OFF • total time: 10-15min Usually 10-15min of sonication is enough, but to be sure you need to try and check it yourself.
