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Can you probe with less than 18.75 ug of cRNA?

April 26, 2017crna probe

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Can you probe with less than 18.75 ug of cRNA?

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The Core has conducted hybridizations with as low as 2.5 ug of cRNA with some systematic deviations as compared to 18.75 ug of cRNA. We recommend 18.75 ug; it is your decision. If you decide to use lower or higher amounts you should keep that consistent among all of your experiments if you plan on comparing chips from different experiments.