Can you please explain the logic behind chromosome walking. In what experimental cases would it be better to use chromosome walking than normal sequencing?
Chromosome walking allows you to find an ordered set of fragments of DNA that are physically near a known marker. Sequencing provides the order of nucleotides within a defined segment of DNA. As such the two techniques are fundamentally different but may overlap. For instance, (1) one often sequences the fragments one obtains from chromosome walking to identify genes. (2) one can sequence an entire chromosome (a laborius, multi-lab, multi-year task) and obviate the requirement for chromosome walking.
