Can you observe cells on cell culture membrane when the insert is inside of the MultiWell Dish?
Due to the large pore size on the polycarbonate inserts, spots or holes are observed which represent the membrane. On this plane, the most visible cells are located on the membrane, whereas the slightly blurred backdrop may be the cells on the dish which is a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of this membrane. However, the cells are visible in culture. Due to the very small pore size of the Anopore membrane, one must need to focus through several planes of view to distinguish between the cells on the membrane vs. those on the dish. This can be achieved by focusing up and down on an inverted microscope. Those cells that are visible first (the objective is the furthest distance from the sample) are on the plate. The cells viewed second (the objective is closer to the sample) are on the membrane. This membrane provides better resolution for photography and observing cells in culture.
