Can you make the oligos having a high percentage of G residues?
It is known that oligos having a high percentage of ‘G’ residues are difficult to synthesize, especially if the sequence contains a run of ‘G’. It is also reported if there is a run of four or more ‘G’, oligos tend to aggregate and form “guanine tetraplex”. (Poon and MacGregor, Biopolymers, 1998, 45, 427-434.) By substituting inosine for some of the ‘G’, the formation of “guanine tetraplex” can be disrupted.
It is known that oligos having a high percentage of G residues are difficult to synthesize, especially if sequence contains several G in a row. It is also reported if there are Gs existed four or more in a row, oligos tend to aggregate and form guanine tetraplex. (Poon and MacGregor, Biopolymers, 1998, 45, 427-434.) By substitution of inosine for some of G, the formation of guanine tetraplex can be disrupted.
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