Can you make phosphospecific antibodies? Are there special requirements or problems?
Phosphospecific monoclonal antibodies can be somewhat more difficult to generate than conventional anti-peptide antibodies and require additional screening. On the other hand, the technology is not different from conventional methods of generating monoclonals, and some phosphoepitopes are quite immunogenic. It is sometimes difficult to get phosphospecific antibodies that do not show at least limited cross reactivity with phosphoepitopes on other proteins. This is presumably because a key element of the epitope (ie, the S, T, or Y phosphate group) is common to many other phosph-proteins. Although these issues present special challenges, the overall success rate is reasonable, and these antibodies can be extraordinarily valuable in signaling studies.
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- What are the special requirements/suggestions for students with chronic medical or psychological problems?
