Can you give us an overview of the procedural steps that our samples will undergo during a ChIP/DNA IP assay at Genpathway?
The process will vary slightly depending on the samples sent and assay being performed. In general, the steps are: • Samples are processed to isolate the chromatin or DNA (also includes sonication). The resulting chromatin or DNA isolates are quantified and the yields are sent to the customer. • Antibody tests (using Genpathway’s Query (QPCR) assay or hybridization to a tiling array) are performed as needed using one or several of the customer samples. Results are sent to the customer and additional antibodies are tested as needed. • ChIP/DNA IP reactions are carried out with the chromatin or DNA, followed by reversal of cross-links (for chromatin) and isolation of the resulting enriched ChIP/IP DNA. • ChIP/DNA IP reactions are QC’ed using Genpathway’s Query (QPCR) assay to measure signals at positive regions expected to be bound by the factor in comparison to negative control regions. • If the samples are to be tested by ChIP Sequencing or ChIP-on-Chip, the ChIP/IP DNAs are amplified
