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Can we compare the intensity of different protein spots and relate that to their abundance?

April 26, 2017Abundance compare different intensity protein relate spots

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Can we compare the intensity of different protein spots and relate that to their abundance?

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Within a single gel it is not possible to compare the intensity of different protein species. Fluorescence can become non-linear for some highly abundant spots and when using broad-range IPG strips, many spots have overlapping boundaries. Neither of these phenomena effect quantitation between different images in the same gel (i.e. for 2-D DIGE experiments) but they do prevent accurate quantitation between spots in the same image.