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Can this enzyme be used in digestion protocols with Tris acetate buffer instead of Na Acetate? Will it poison or even work?

April 26, 2017acetate buffer digestion enzyme Na Poison protocols Tris Used

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Can this enzyme be used in digestion protocols with Tris acetate buffer instead of Na Acetate? Will it poison or even work?

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Heparitinase has 80% activity in 50 mM Tris-Acetate buffer at pH 7.0 + 5mM Ca-Acetate, compared with sodium acetate buffer of pH 7.0 I think you can use Tris-acetate buffer if you don’t care about going down in enzyme activity 20%.