Can the virus be stored in the elution buffer – what are the storage conditions? Does anything have to be added?
• The elution buffer contains high (>1M) salt/Tris-HCl (pH 8), and should be buffer exchanged (w/2 spin protocol) with the Amicon Ultra before using for their application or addition of glyerol for long-term storage. If it is not buffer exchanged, the tube could form salt crystals and might not freeze properly. Also, if kept in the elution buffer, their downstream applications (i.e. with cells) might be adversely impacted.
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