Can the ViroScoreTM system accept and understand IUPAC since most sequence that come out from trugene will retain the ATGC and W R, etc?
Yes we handle ambiguous codons in ViroScoreTM Suite (Trugene or any sequencing platform). We generate all possible amino acids and use them for resistance interpretation. We were the first to do it in online HIV resistance interpretation in 2001. Mutations coming out from ambiguous codons are indicated with * in the list of mutations of the ViroScoreTMr report.
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