Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can the Transcreener ADP Assay be used for ATPase drug development?

April 26, 2017ADP assay Development drug Used

0

Posted

Can the Transcreener ADP Assay be used for ATPase drug development?

1 Answer

0

Posted

Yes. The Transcreener™ ADP Assay was developed to follow the progress of kinase and ATPase reactions. Kinases catalyze the transfer of a phophate group from ATP to a protein, peptide, lipid or small molecule substrate generating ADP, the invariant product. During ATPase reactions, the transfer of a phosphate group from ATP to a water molecule generates ADP, and thereby competes with the ADP tracer for binding to the monoclonal anti-ADP antibody. See BellBrook poster presentation for data on Hsp70 and Hsp90 ATPases using the Transcreener™ ADP Assay.