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Can the sensitivity be increased by altering the conjugate concentration?

April 26, 2017altering concentration conjugate increased

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Can the sensitivity be increased by altering the conjugate concentration?

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Use the recommended concentration of anti-Phospho-Tyrosine-HRP Detection Antibody. Using a higher concentration of detection antibody may result in high background. Longer exposures to X-ray film (5 to 10 minutes) should increase sensitivity.