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Can the primary antibody be incubated overnight in the SNAP i.d. System?

April 26, 2017Antibody incubated overnight primary snap system

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Can the primary antibody be incubated overnight in the SNAP i.d. System?

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• The protocol for the SNAP i.d. System is designed to enable rapid immunodetection, overnight antibody incubation should not be necessary. However if overnight incubation is desired we recommend removing the blot from the blot holder after blocking and incubating the blot under the standard condition (e.g., standards antibody concentration in a rocking tray). The next morning re-assemble the blot in the blot holder and continue with the SNAP i.d. immunodetection.