Can the Octet System read crude samples?
BLI readings using the Octet System are based on the proteins bound at the tip of the biosensor and are independent of the sample medium for most biological applications. Quantitation and kinetic measurements can be made in serum-containing media (up to 10%) DMSO-containing buffers, periplasmic fractions, uncleared cell culture supernatants, and uncleared bacterial lysates.