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Can the molecule be bound but not recognised during the detection?

April 26, 2017bound detection molecule recognised

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Can the molecule be bound but not recognised during the detection?

1 Answer

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10 Posted

Yes. If lysine or cysteine is a part of the epitope recognised by the antibody then a binding of these amino acids to the photoprobe will lead to a reduced recognition and a lower signal. An increase in the peptide concentration could solve this problem.