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Can the kit be used for nuclear preparations that were derived from frozen samples?

April 26, 2017derived frozen kit nuclear preparations samples Used

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Can the kit be used for nuclear preparations that were derived from frozen samples?

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If the experimental tissue was not frozen in a cryopretective medium, it is possible that the nuclei will be fragmented. Thus, Diagenode recommends working with fresh tissues. Samples can be frozen after nuclei lysis and chromatin shearing at -80°C for several months and yield good results. Alternatively it is possible to fix the tissue and then to freeze the samples.