Can the intein-CBD enhance solubility?
Based on the tests with the IMPACT system, there appears to be no direct correlation between enhanced solubility and the intein-CBD fusion construct. However, there have been a few examples of proteins that have been solubilized when fused to the intein-CBD fusion. For example, a plant protein was solubilized after being expressed as a three part fusion with the intein and CBD. In another case, the introduction of an extra Gly residue (which is the native residue upstream of the intein) between the protein of interest and the Sce VMA1 intein has also been shown to improve the solubility of the fusion protein. Furthermore, the addition of a nonionic detergent to the column buffer (0.1-0.5% Triton X-100 and 0.1% Tween 20), or higher salt concentration (up to 2 M NaCl) may increase the solubility. Also, since lower temperatures should lessen aggregation, it is recommended to try an induction at 15°C overnight. We now offer many IMPACT vectors containing different inteins and allowing for
