Can the IMPACT System be used to pull out proteins from E. coli or mammalian cell extracts which react with the target protein?
Since proteins are purified in a single chromatographic step under native conditions, it is possible to use the IMPACT System to perform the proposed experiment. The protein complex may be released by induction of the intein-mediated cleavage reaction. Otherwise the uncleaved fusion protein and the bound protein may be stripped by 0.3 M NaOH.
Related Questions
- Can the IMPACT System be used to pull out proteins from E. coli or mammalian cell extracts which react with the target protein?
- What are the differences between Cell culture (used in MFIII) and Biological Extract (as found in most placenta Extracts?
- What are the differences between Cell culture (used in ) and Biological Extract (as found in most placenta Extracts?
