Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can the Diagenode MeDIP kits be used with DNA obtained from paraffin-embedded samples?

April 26, 2017diagenode dna Kits medip obtained paraffin-embedded samples Used

1

Posted

Can the Diagenode MeDIP kits be used with DNA obtained from paraffin-embedded samples?

1 Answer

0

Posted

Diagenode has not tested its MeDIP kits for immunoprecipitating DNA from paraffin-embedded samples. It should be possible to perform MeDIP but note, that the quality of DNA from paraffin-embedded samples is not optimal for MeDIP. The fixation introduces DNA modifications (addition of mono-methyl group, formation of bridges between adjacent bases, generation of apurinic and apyrimidinic sites and strand breaks). DNA quality varies from sample-to sample. However, our customers have successfully used the antibody anty-5methyl cytidine (the same as used in our MeDIP kits) in immonohistochemistry assay on fixed sections.