Can the coverslip be removed from the glass bottom dish?
Yes. However, for most applications, cells growing in the glass bottom dish can be viewed without removal of the coverslip. If necessary (e.g. for long term storage purposes), the coverslip can be removed using the following procedure: 1. Order Part # PDCF OS 30 (Dow Corning fluid OS 30, available from MatTek) 2. Invert the cover of the dish. 3. Pipette 1.0 ml of OS 30 into the inverted cover. 4. Place the bottom of the dish in the cover. Make sure that the liquid is touching the bottom of the coverslip. 5. Allow the dish to sit in the OS 30 for 45 minutes at room temperature. 6. Dry the bottom of the coverslip with an absorbent paper towel. 7. Place the dish on a clean surface. Using forceps, press down on the edge of the coverslip to separate the coverslip from the dish. Note: If the above procedure is followed, the PDCF OS 30 liquid will not contact the cells and will not disrupt cells on the coverslip or the staining thereof.
Yes. However, for most applications, cells growing in the glass bottom dish can be viewed without removal of the coverslip. If necessary (e.g. for long term storage purposes), the coverslip can be removed using the following procedure: 1. Order Part # PDCF OS 30 (Fluid for removal of coverslips from glass bottom dishes) 2. Invert the cover of the dish. 3. Pipette 1.0 ml of fluid into the inverted cover. 4. Place the bottom of the dish in the cover. Make sure that the liquid is touching the bottom of the coverslip. 5. Allow the dish to sit in the fluid for 45 minutes at room temperature. 6. Dry the bottom of the coverslip with an absorbent paper towel. 7. Place the dish on a clean surface. Using forceps, press down on the edge of the coverslip to separate the coverslip from the dish. Note: If the above procedure is followed, the PDCF OS 30 fluid will not contact the cells and will not disrupt cells on the coverslip or the staining thereof.
