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Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

April 26, 2017bacterial miniprep Pellet pipetting procedure quicklyse resuspended Shortening time vortexing

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Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

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No. Vortexing for 30 seconds as described in the QuickLyse Miniprep Handbook is absolutely critical to resuspend and lyse the bacterial cells. Failure to follow this step will result in greatly r…