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Can the 2D DIGE experiment dedect phosporylation?

April 26, 2017dige experiment

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Can the 2D DIGE experiment dedect phosporylation?

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Yes. When proteins are phosphorylated, the protein pI will shift to a more acidic region and it can be easily detected by 2D DIGE. For identification of phosphorylation site, you will need more protein and different methods such as phospho-peptide enrichment, LC-MS/MS etc.